Lab Report Example | Topics and Well Written Essays - 2250 words

Lab Report Example Invention of polymerase chain reaction and thermo stable DNA polymerase has revolutionized this concept. Many techniques like Variable tandem repeat (VNTRs) and Short Tandem Repeat(STRs) based Amplified Fragment Length Polymorphism(AFLP) or DNA fingerprinting, Gender ID (Amelogenin), Mitochondrial D-Loop DNA for Maternal identification etc. have been developed for forensic investigations. PM&DQA1 is the first PCR based typing system, developed by the scientist at Cetus Corporation and commercialized by Applied Biosystems. Inc. PM& DQA1 is simple and rapid technique mainly employed in case of limited DNA sample or in case of highly degraded DNA. The test is performed on 6 different loci to investigate pleomorphism using hybridization based Reverse DOT-BLOT methodology. The major limitation of this technique is its differentiating power due to limited diversity found among 6 loci among different human population. Crime investigation obtained new direction after invention of DNA based techniques for identification of criminals. The biggest advantage of DNA based techniques is the requirement of very small amount of biological samples which includes blood, hair, semen etc. Invention of polymerase chain reaction (PCR) and thermo stable DNA polymerase has revolutionized these DNA based techniques due to its immense power of DNA multiplication. There are many DNA based techniques that are employed for detection of crime and criminals including Restriction Fragment Length Polymorphism (RFLP), Variable tandem repeat (VNTRs) and Short Tandem Repeat(STRs) based Amplified Fragment Length Polymorphism(AFLP) or DNA fingerprinting, Gender ID (Amelogenin), Mitochondrial D-Loop DNA for Matrilineal identification. Criminal identification based on human body parts or component has been used since last century as part of forensic investigations. The classical example being Human finger prints which was recogn ized by British physician Sir Francis Galton (1). In the beginning of 20th century many physiologists and doctors realized that blood cell has distinct antigens and they are different from individual to individuals. Initially blood cell antigens were taken into consideration only in case of blood transfusion and surgery but later on its forensic utilities were realized and it was employed to solve problem of paternity dispute, child mix-up etc. Those days blood samples saliva and semen samples were produced in court as evidence against criminals but it was not taken as conclusive evidence due to its limited distinguished power. In 1970, the things started to change after discovery of DNA structure and the increases in the understanding of its variability between individual to individual. Once DNA was established as target molecule for forensic investigation, many researchers came forward with different methodology and different locus having higher variability like micro and mini sat ellites. Initially there were many questions that were raised over methodologies and interpretation of DNA based identification systems. But thorough investigation and population genetics based

Responsive Essay Example | Topics and Well Written Essays - 500 words

Responsive - Essay Example He has stayed amongst the Kung Bushmen of the Kalahari Desert for a number of years, but he barely knows them in totality. The best time that he learns something substantial about their behavior is when he has only one Christmas to celebrate in their land. This is a group of people that are of a funny character. They make us marvel at values of their society. These include their togetherness that they uphold in the society. In so many ways, they used to enjoy in unity through sharing their hunt almost in each and every single day. The story in this article exposes the true nature of the Kung Bushmen. As a matter of fact, they are a group of people in the society that enforces humility in people who do not have it at hand. They try as much as possible to resist pride that may crop up in someone after a little achievement of victory. For instance, it is seen in the text how people hunted and brought fatty meat at home, but in rejoinder received the least of appreciation. The anthropologist himself wondered why gratitude was barely shown to those who went hunting and gathering. To set the facts right, a member of the Kung Bushmen community gives a hidden answer, all about arrogance! This is what I realize that the Bushmen Community try as much as possible to resist for its existence would only lead to a feeling of superiority over the others. In short, members of society at large prefer to be equal to one another. In this article, what comes out more vividly is mockery of the highest order. In the first instance, it took a lot of sacrifice for Ontah, the anthropologist to have the fattest ox for his last Christmas celebration amongst the Kung. In return, what he receives before that big day is ridicule and travesty. It is amazing how each person confronts him with a similar scorn. Exposing his folly, he hardly realizes the pure intentions of these people and to make him do, he has to inquire and seek judgment from a few